Journal: bioRxiv

Article Title: Topographic CA1 input shapes subicular spatial coding

doi: 10.64898/2026.03.24.714092

Figure Lengend Snippet: (A) Histology of GRIN lens implantation in the dorsal subiculum of a Ctrl and a Lphn2 cKO mouse. Green: GCaMP6, Blue: DAPI. Right, enlarged view of GCaMP6-expressing subiculum neurons. (B) Property comparisons between place cells in Ctrl and Lphn2 cKO mice (Wilcoxon rank-sum test: all p > 0.05). (C) Three representative place cells in the shuttle box environment from three Ctrl mice, plotted as in . (D) Same as C, but for Lphn2 cKO mice. (E) Place cell remapping between the left and right shuttle box compartments (Wilcoxon rank-sum test: p > 0.05). (F) Illustration showing miniscope image alignment to ensure a consistent axis across different mice.

Article Snippet: The GRIN lens (0.25 pitch, 0.55 NA, 1.8 mm diameter and 4.31 mm in length, Edmund Optics) was then slowly lowered with a stereotaxic arm to the subiculum to a depth of −1.75 mm relative to the measurement of the skull surface at bregma.

Techniques: Expressing

Journal: bioRxiv

Article Title: Topographic CA1 input shapes subicular spatial coding

doi: 10.64898/2026.03.24.714092

Figure Lengend Snippet: (A) Schematic of miniscope calcium imaging in the subiculum. (B) Open arena environment shapes. (C) Two representative place cells from two control (Ctrl) mice. Each column represents a single place cell, with its activity tracked across multiple sessions. The raster plot (left) shows deconvolved spikes (red dots) overlaid on the animal’s running trajectory (grey lines), while the spatial rate map (right) is color-coded, with red indicating the maximum and blue indicating the minimum values. (D) Same as C, but for Nts-Cre;Lphn2 fl/fl ( Lphn2 cKO) mice. (E) Comparison of mean spike rate across all recorded neurons from Ctrl and Lphn2 cKO mice. Each dot represents a mouse (Wilcoxon rank-sum test: p = 0.045; Ctrl: n = 10 mice (total 1782 neurons); Lphn2 cKO: n = 11 mice (total 2749 neurons)). The black bar represents the mean. Quantification was based on day 3 square session. (F) Same as E, but for the information score comparison (Wilcoxon rank-sum test: p = 0.084). (G) Comparisons of place cells properties in Ctrl and Lphn2 cKO mice (Wilcoxon rank-sum test: all p > 0.05). (H) Illustration of the anatomical structure under the miniscope imaging window. The circle represents the implanted GRIN lens (1.8 mm diameter). The red box represents the field of view of the miniscope CMOS sensor, which is 700 μm × 450 μm. PrS: presubiculum; dhc: dorsal hippocampal commissure. (I) Left: anatomical footprints of all recorded subicular neurons from a representative Ctrl mouse in a single session. Each grey contour represents a neuron. Right: corresponding density map, with neuron density ranging from low (black) to high (white). Spatial bin size: 18 × 18 μm. (J) Left: distribution of identified place cells (red) in the subiculum from the same mouse as in I. Right: density map of place cells, normalized by the density of all neurons. (K) Same as I, but for an Lphn2 cKO mouse. (L) Same as J, but for an Lphn2 cKO mouse. (M) Left: place cell density map, averaged across all recording sessions (square, circle, and shuttle box), from a representative Ctrl mouse. Right: same as left, but for a representative Lphn2 cKO mouse. (N) Distribution of place cell density along the proximodistal axis for all Ctrl (left) and Lphn2 cKO (right) mice. Each row represents a mouse, with a red dot indicating the peak of its distribution. (O) Comparison of place cell density distribution along the proximodistal axis between Ctrl and Lphn2 cKO mice (two-way ANOVA with Sidak’s multiple comparisons: * p-values range from 0.029 to 0.045). (P) Peak location of place cell density distributions (Wilcoxon rank-sum test: p = 0.013). (Q) Same as O, but along the caudal-to-rostral axis (two-way ANOVA with Sidak’s multiple comparisons: all p > 0.05). (R) Same as P, but along the caudal-to-rostral axis (Wilcoxon rank-sum test: p = 0.46).

Article Snippet: The GRIN lens (0.25 pitch, 0.55 NA, 1.8 mm diameter and 4.31 mm in length, Edmund Optics) was then slowly lowered with a stereotaxic arm to the subiculum to a depth of −1.75 mm relative to the measurement of the skull surface at bregma.

Techniques: Imaging, Control, Activity Assay, Comparison